Fig. 4: Lipidomic analysis of TgFLP12 revealed a general decrease in the composition of myristic acid.

a FA total amount, quantified by GC-MS is unchanged after iΔTgFLP12 repression. Data are presented as mean values +/− SD (n = 4). b FA composition in Mol% of TgFLP12 lacking parasites ( + ATc) compared to parasites harboring TgFLP12. Two-way Anova, Šídák’s multiple comparisons, data are presented as mean values +/− SD (n = 4). c Lipid composition revealed by one-dimensional TLC and GC-MS showed a decrease in phospholipids and an increase in neutral lipid TAG and DAG in parasites without TgFLP12 (+ATc) compared to parasites with TgFLP12, one sample t test, data are presented as mean values +/− SD (n = 4). Major FA relative abundance of d triacylglycerols TAG, e diacylglycerols DAG, f phospholipids PL, and g free fatty acids FFA. Two-way Anova, Šídák’s multiple comparisons, data are presented as mean values +/− SD (n = 4). h Relative abundance of the different lipid classes made from scavenged lipids revealed that neutral lipids TAG and FFA from scavenged lipids increase after addition of ATc. One sample t test, data are presented as mean values +/− SD (n = 4).