Fig. 5: cGAS is necessary for SA-β-gal positivity and SASP expression during senescence caused by loss of DNA methylation, and it acts independently of STING.
From: DNA methylation protects cancer cells against senescence
A RT-qPCR on cGAS in HCT116 lines upon Dox/Auxin treatment. N = 3 biological replicates. B Western blotting of cGAS protein in HCT116 lines at the indicated days upon Dox/Auxin treatment. C Scheme of cGAS knockdown experiments. D Total cell numbers at the indicated days upon Dox/Auxin treatment. N = 3 technical replicates. E SA-β-gal staining (left panel) and quantification (right panel) of the indicated lines. N = 5 fields of view. F RT-qPCR results of cGAS and selected SASP genes. G Scheme of STING knockout experiments. H SA-β-gal staining (left panel) and quantification (right panel). N = 5 fields of view. I RT-qPCR results of selected SASP genes. J Western blotting of cGAS in cytoplasmic and nuclear fractions of HCT116 lines at the indicated days upon Dox/Auxin treatment. All scale bars are 50 µm. Data of A, D, E, H are presented as mean ± SD. Data of A are analyzed by one-way ANOVA with Dunnett’s multiple comparisons test. Data of D, E, H are analyzed by two-way ANOVA with Sidak’s multiple comparisons test. Heatmap data of F, I are presented as mean from three technical replicates. In all figures, we use the following convention: **p < 0.01, ***p < 0.001, ****p < 0.0001, ns: non-significant. Source data are provided as a Source Data file.
