Fig. 2: In early infection establishment, multiple B. dolosa clones are transmitted between CF patients.
From: De novo mutations mediate phenotypic switching in an opportunistic human lung pathogen
a A maximum parsimony SNV phylogeny was built from whole-genome sequencing of 805 B. dolosa isolates from autopsy samples of the suspected index case (Patient J), 122 from serial sputum samples of Patients Q and R, and 112 previously published sequences of isolates from Patient J and 13 other patients a decade prior15. All isolates from Patient Q and R are descended from a subset of Patient J’s diversity; a high-resolution phylogeny of this clade (“Methods”) is shown to the right. The time of sampling of each isolate is indicated by color in the rightmost vertical bar, clades within the tree are shaded by patient, and SNVs of interest for transmission inference are indicated by colored shapes. b Potential transmission scenarios between Patients J, Q, and R are diagrammed, showing the need for either more than two transmission events or parallel nucleotide evolution to explain the observed diversity. Arrows point in the direction of B. dolosa transfer and are colored by the SNV in (a) that precedes a transmission.
