Fig. 3: Spatial Localization of Stromal Subtypes.
A RNA ISH for VEGFA/CXCL13, VEGF/PDGFRA and IL-6/VEGFA/PDGFRA combinations in RLN (n = 1), UCD (n = 3) and MCD (n = 4) were evaluated. Markedly increased VEGF and IL-6 in MCD that colocalized with CXCL13 + FDC in follicles and PDGFRA+ stromal cells in interfollicular areas (2nd row). Colocalization of CXCL13 + FDC and VEGF expression in follicles of UCD is noted (3rd row). Co-expression of IL-6, VEGF and PDGFR+ stromal cells in MCD3 is shown (3rd column, purple cells). Co-expression of IL-6 with PDGFR+ stromal cells in MCD4. Inset image shows high magnification. B Stromal subsets identified from snRNA-seq data are shown in CODEX images after integration by MaxFuse. BEC, LEC and PRC are associated with CD31/CD34+ blood vessels and are increased in UCD and MCD. Markedly increased FDC nuclei are closely associated with regions of dense CD21 meshworks. C Left column: Follicles were identified by cell microenvironment (Voronoi plot shown in top) and split into concentric 100-pixel contours (bottom). Stromal subtypes identified from snRNA-seq data were transferred to multiplexed image data and their abundance assessed at each contour layer (middle and right columns). Statistics were aggregated over each follicle per image region (MCD, n = 11; HVCD, n = 9, RLN, n = 3). Error bands represent a 90% confidence interval. Peaks of stromal cells are evident in layers before peaks of BEC and LEC of MCD. MCD is shown in red, UCD in purple and RLN in blue.
