Fig. 5: Spatial transcriptomics of additional cohort of UCD and MCD cases. | Nature Communications

Fig. 5: Spatial transcriptomics of additional cohort of UCD and MCD cases.

From: Spatial and single cell mapping of castleman disease reveals key stromal cell types and cytokine pathways

Fig. 5: Spatial transcriptomics of additional cohort of UCD and MCD cases.The alternative text for this image may have been generated using AI.

Spatial transcriptomic profiling was performed on tissue microarrays of CD samples from 13 cases using the 10x Xenium platform. A Cell annotations and microenvironment analysis of an iMCD and UCD case are shown. Attenuated follicular (Fl) and expanded interfollicular (iFl) plasma cell and stromal microenvironments are noted in MCD. B Bar plots show relative cell proportions in subsets of RLN, MCD, and UCD. Expanded stromal and plasma cell subsets are noted. C UMAP visualization of stromal subtypes highlights distinct compositional differences between iMCD and UCD. Density projections illustrate differential expansion of BRC and TRC in UCD and MCD, respectively. BEC are expanded in both UCD and MCD (D). Elevated IL-6 module score (IL6, LIFR, OSMR) and VEGFA expression levels in iMCD cases. E DLL4^NOTCH1 interaction was evaluated in the Xenium data and showed a disease and distance dependency consistent with physical interaction. Source data are provided as a Source Data file in supplementary dataset.

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