Fig. 4: Roles of active site residues Tyr-61 and Lys-71 in nucleotide incorporation.
From: Visualizing DNA polymerase ι catalyze Hoogsteen-directed DNA synthesis
(A) Nucleotide-incorporation activity of the wild-type, mutant Y61A, and mutant R71A pol ι proteins. These experiments were repeated four times to ensure reproducibility. The uncropped gel images are provided as a Source Data file. (B) Close-up of the active site of the mutant R71A substrate complex with the newly formed base pair labeled n, the primer-terminal base pair labeled n-1, and the base on the 5′ side of the template base labeled n + 1 and with a polder map contoured at σ = 3.0 showing the weak electron density corresponding to the incoming dTTP (green mesh).
