Fig. 4: Real-time trafficking of the endocytic organelle maturation by BLINK nanoprobe.
From: Cascade-heterogated proton nanotransistors for multiplex pH-interval imaging
a representative fluorescent images of BLINK at different pH values in live Panc02 cells. The cells were treated with nigericin (10 μM) and valinomycin (10 μM) in the PBS buffers with different pH values. Red (BP), FRET-Cy3.5 excited by 405 nm; green (LP), Cy3.5 excited by 561 nm. b representative confocal and fast FLIM images of BLINK in live cells at single-organelle resolution. Panc02 cells were chased at 30 min after 10-min pulse with BLINK [6.1, 6.5]. Phasor analysis was applied to measure fluorescence lifetime of DEAC and the FRET efficiency between DEAC and Cy3.5. Blue, DEAC; red (BP), FRET-Cy3.5; c fast FLIM images of DEAC at different chase time for analysis of the endosome maturation. The fluorescence lifetime of DEAC increased as endosomes maturation. FLIM: λex = 440 nm, λem = 450-520 nm.
