Fig. 5: BLINK as endosome-trackers compared with traditional probes and biomarkers.
From: Cascade-heterogated proton nanotransistors for multiplex pH-interval imaging
a representative confocal images of live cells co-incubated with BLINK[6.1, 6.5] (100 μg mL−1) and LysoTracker deep red (10 nM). Colocalization analysis at single-organelle resolution showed the threshold pH of LysoTracker deep red for acidic organelles imaging. Red (BP), FRET-Cy3.5 excited by 405 nm; green (LP), Cy3.5 excited by 561 nm; blue, LysoTracker deep red excited by 633 nm. b representative confocal images showed colocalization of BLINK[6.1, 6.5] (red) with different endocytic organelles, including early endosomes (Rab5a-GFP, green), late endosomes (Rab7a-GFP, green) and lysosomes (LAMP1-GFP, green). c frequency of colocalization between BLINK[6.1, 6.5] with biomarkers from (b). d representative confocal images showed colocalization of BLINK[5.3, 5.7] (red) with different endocytic organelles (green). e frequency of colocalization between BLINK[5.3, 5.7] with biomarkers from (d). Source data are provided as a Source Data file.
