Fig. 5: MHs are usually selected by Pol θ within 15 nucleotides of the 3ʹ end of ssDNAs.

a End-joining read count percentage for insertions, deletions and complex event outcomes for the indicated top oligo libraries with a blocked bottom oligonucleotide (Oligo 10). Each top oligo library (4 bp MH + 0–5 nt) contains 1, 4, 16, 64, 256, and 1024 individual oligonucleotide outcomes, respectively. Dots indicate the individual read counts, and the box plot shows the distribution. Box plots show the median (center line), the 25th and 75th percentiles (bounds of box), and Tukey whiskers (1.5X interquartile range). The statistical analysis was performed using a paired two-tailed t-test. Significance is indicated as follows: ns (not significant, p > 0.05), *(p ≤ 0.05), ** (p ≤ 0.01), *** (p ≤ 0.001), and **** (p ≤ 0.0001). b Definition of MH anchoring position for the extended top oligonucleotide and bottom oligonucleotide template. Arrow indicates direction of Pol θ extension. The anchoring position was defined as the distance (number of bases) between the 3′ ends of the joining ssDNAs. The assigned base pairing code for this example is labeled. Codes for all pairing types are described in Supplementary Data 1. A 6 nt MH region is boxed in a green square. c MH anchoring patterns of different oligo pools with a blocked bottom oligonucleotide (Oligo 10). Read count percentages were plotted with anchoring positions. d Read count percentage of MHs anchored at different regions for the indicated oligo libraries with a blocked bottom oligonucleotide (Oligo 10). Source data are provided as a Source Data file.