Table 1 Analysis of microhomologies (MH) used for deletions at DSB target sites^a

Figure	Deletion size	Deletion sequence and cleavage site^b	Previously^a identified MH	Redefined MH^c
DSB target site 1: CCAGTGCAGGCTGCCTATCAGA ▿ AAGTGGTGGCTGGTGTGG
Fig. 8c	4 bp	AGA ▿ A	AG	XAGX
Fig. 8d	21 bp	AGTGCAGGCTGCCTATCAGA ▿ A	AGTG	AGTGXXGGCTG
	16 bp	AGGCTGCCTATCAGA ▿ A	AG	AG
	7 bp	GA ▿ AAGTG	G	AXXXG
Fig. 8c	4 bp	CAGA ▿	0	XAGX
DSB target site 2: GGTCTGTGTGCTGGCCTATC ▿ ACTTTGGCAAAGAATTCACCCC
Fig. 8b	13 bp	TGGCCTATC ▿ ACTT	TGGC	TGGCXXA
Fig. 8b	13 bp	ATC ▿ ACTTTGGCAA	A	TGGCXXA
	6 bp	CTATC ▿ A	CT	CTXTG
	18 bp	CTGTGTGCTGGCCTATC ▿ A	CT	CTXTG
DSB target site 3: CCTGGCCCACAA ▿ GTATCACTAAGCTCGCTT
Fig. 8a	10 bp	CCACAA ▿ GTAT	C	CXXXAA
Fig. 8a	10 bp	AA ▿ GTATCACT	AA (or AAG)^d	CXXXAA
	16 bp	CCTGGCCCACAA ▿ GTAT	C	TXXC
	9 bp	CACAA ▿ GTAT	CAC	CACXA
	9 bp	A ▿ GTATCACT	A	CACXA
	7 bp	CAA ▿ GTAT	CA	CA
	4 bp	AA ▿ GT	A	A
DSB target site 4: CCTGGCCCACA ▿ AGTATCACTAAGCTCGCTT
	7 bp	CA ▿ AGTAT	CA	CA
	9 bp	CACA ▿ AGTAT	CAC	CAC
	16 bp	CCTGGCCCACA ▿ AGTAT	C	AXTXXC

^aTarget sites, deletion size and microhomologies from Fig. 6D and Supplementary Fig. 4 of ref. ¹².
^bThe Cas9 cleavage site is marked by ▿.
^cX indicates a mismatch in the predicted interrupted microhomology, see Fig. 8 for examples.
^dIdentified as microhomology AAG in ref. ¹², but likely intended as AA because the G lies on the opposite side of the Cas9 cleavage site.

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