Table 3 plasmids and strains

From: Cryo-EM structures of the E. coli Ton and Tol motor complexes

plasmid

ptolAstrep

tolA N-term streptag-II, pCDFDuet, Smr

this study

ptolAstrep-TEV

ptolAstrep TEV site, pCDFDuet, Smr

this study

pQR

ybgC-tolQ-tolR operon, pT7-1QR, Ampr

Germon et al., 199820

ptonBstrepII

tonB C-term TEV site and streptag-II, pACYCDuet, Cmr

this study

pexbB

exbB, pET26b, Kanr

Celia et al., 201610

pexbD10his

exbD, C-term TEV site and 10his-tag, pCDFDuet, Smr

Celia et al., 201610

strain

W3110

F’λ-IN(rrnD-rrnE)1 rph-1

Bachmann, 197241

∆tolA

W3110 ∆tolA::frt

Petiti et al., 201942