Fig. 1: Generation of ABab-I mice by PiggyBac transposon system.
From: Mice with a diverse human T cell receptor repertoire selected on multiple HLA class I molecules
Schematic representation of PiggyBac (PB) transposon strategy for the generation of ABab-I mice using pronuclei microinjection technology. The PB targeting ABab-I transgene (flanked by ITRs for PB transposase catalysis) has repeating elements of six HLA ORFs constructed one after another (5′-to-3′) each with its own promoter and 3′-UTR components. Each HLA ORF is a human-mouse fusion monochain containing α1 and α2 heavy chain cDNA regions from respective human HLA alleles, while α3, transmembrane (TM), and cytoplasmic domain (CYT) from murine H-2 Db gene fused by a glycine-serine linker to human ß2 microglobulin (ß2M). Created in BioRender. Dhamodaran, A. (2025) https://BioRender.com/wii3mup.
