Fig. 2: T cell development in ABab-I, ABab-A2, and C57BL6/N mice.

A Representative flow cytometry analysis of thymocyte staining from ABab-I, ABab-A2, and C57BL6/N mice (n = 5). 1st lane: CD4⁺ and CD8⁺ single- and double-positive cells, gated on total thymocytes; 2nd lane: cell percentages of CD5⁺CD69⁺ cells, gated on lymphocytes; 3rd lane: percentages of CD3⁺ cells; 4th lane: CD4⁺ and CD8⁺ single- and double-positive cells gated on CD3⁺ cells. B Quantified cell numbers of thymocyte populations: CD8SP, DP, CD5CD69, and CD3CD8 in respective mouse strains. C Quantified percentages of thymocyte populations: CD8SP, DP, DN, and CD4SP in respective mouse strains. D Representative flow cytometry analysis of splenocyte staining from ABab-I, ABab-A2, and C57BL6/N mice (n = 5), gated on CD3⁺ lymphocytes. 1st lane: CD8⁺CD44^high cells; 2nd lane: CD4⁺CD44^high cells. E Quantified percentages of CD44^high CD4⁺ and CD8⁺ populations from ABab-I, ABab-A2, and C57BL6/N mice (n = 5). Data in (B), (C), and (E) are presented as mean ± SD. Multiple group statistical comparisons were performed by one-way ANOVA, followed by pairwise comparisons using two-tailed unpaired Student’s t-test. P values indicate: *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001; ns not significant. Exact P values and source data are available in the Source Data file.