Fig. 4: Phenotypic characterization of peripheral T cells in ABab-I mice.
From: Mice with a diverse human T cell receptor repertoire selected on multiple HLA class I molecules
A Peripheral blood cells from young (8–12 weeks) indicated mouse strains were stained with antibodies specific for CD3, CD8, and CD4 chains and analyzed by flow cytometry (gated on CD3+ lymphocytes). One representative plot out of multiple experiments is shown (n > 30). B Absolute CD3+CD8+ T cell numbers/µl blood and (C) Absolute CD3+CD4+ T cell numbers/µl blood. Summarized data from C57BL6/N (n = 9), ABab-A2 (n = 32), and ABab-I (n = 60) mice. D CD8/CD4 ratio based on absolute numbers in peripheral blood from C57BL6/N (n = 11), ABab-A2 (n = 12), and ABab-I (n = 11) mice. E Spleen and lymph nodes from young (8–12 weeks) indicated mice were stained with antibodies specific for CD3, CD8, and CD4 chains and analyzed by flow cytometry (gated on CD3+ lymphocytes). One representative plot out of multiple experiments is shown (n > 10). F Absolute CD3+CD8+ T cell numbers/mouse and (G) Absolute CD3+CD4+ T cell numbers/mouse. Summarized data from ABab-A2 (n = 7) and ABab-I (n = 5) mice. H CD8/CD4 ratio based on absolute numbers in lymphoid organs from ABab-A2 (n = 12) and ABab-I (n = 5) mice. Each data point in scatter plots represents an individual mouse from indicated strains. Data in (B–D), (F–H) are presented as mean ± SD. Multiple group statistical comparisons in (B–D) were assessed by one-way ANOVA, followed by pairwise comparisons between ABab-A2 and ABab-I groups using two-tailed unpaired Student’s t-test. P values indicate: ***P < 0.001 and ****P < 0.0001; ns not significant. Exact P values and source data are available in the Source Data file.
