Fig. 5: Sex-determining region of M. rotundifolia.

a Linkage disequilibrium as the mean r2 per kbp. The gray triangles on the scale mark the position of the sex-linked markers in muscadines, S2_4635912 and S2_508598321. b Whole-sequence alignments of the F (top) and M (bottom) haplotypes against the F haplotype of M. rotundifolia Trayshed. c Schematic representations of the gene and intergenic repeat contents in the sex-determining region (SDR) of the males M. rotundifolia Trayshed and DVIT1750, and the female muscadine Fry. The symbols ♀ and ♂ represent the female and male flower sex type of the muscadine individuals. For each haplotype, genes are represented with arrows and repeats with rectangles. White arrows depict genes that are not part of the Vitis SDR. The black triangles mark the position of the breaking points of the inversion in the M haplotype compared to the F haplotype. Pseudogenes are indicated with an X. Gene content representations from (a) and (c) share the same legend. Source data are provided as a Source Data file.