Fig. 6: Spironolactone inhibits mineralocorticoid receptor (MR) binding on the neutrophil gelatinase-associated lipocalin (NGAL) in TGF-β1-activated smooth muscle cells (SMC). | Nature Communications

Fig. 6: Spironolactone inhibits mineralocorticoid receptor (MR) binding on the neutrophil gelatinase-associated lipocalin (NGAL) in TGF-β1-activated smooth muscle cells (SMC).

From: Mineralocorticoid receptor activation contributes to intestinal fibrosis through neutrophil gelatinase-associated lipocalin in preclinical models

Fig. 6

a, b Murine SMC were incubated with aldosterone (100 nM), spironolactone (10 µM) in response to TGF-β (10 ng/mL) for 24 h (n = 4 from independent experiments). a Relative mRNA expression of Cola1 and Col3a1 from SMC cells lysates (n = 4 per group; Kruskal-Wallis test with Dunn’s multiple comparisons test). b Chromatin immunoprecipitation (ChIP) followed by qPCR analysis (ChIP-qPCR) performed with an anti-MR antibody in SMC upon of TGF-β1 and spironolactone stimulation (n = 5 per group; Friedman test with Dunn’s multiple comparisons test). Data are presented as mean values +/− SEM.

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