Fig. 3: Heterogeneity and dynamics of cellular senescence in aging human muscle.

A–D Discriminative dimensionality reduction (DDR) tree visualization of MuSC trajectory with mapping of pseudotime (A), age group (B), senescence annotation (C), and CDKN1A expression level (D). E Plot showing the ss-GSVA score of cell cycle activity signature genes along MuSC aging trajectory psuedotime. The solid yellow line is the local regression result for individual pseudotime bins (55 total, sized 0.10 per bin), with the gray shadow depicting the 95% CIs. F Heat map visualization of expression levels of genes (right) with correlated expression profiles to MuSC aging pseudotime ordered from Early to Late stage. Left: row annotation showing the functions of the genes. G Heat map visualization of expression levels of DEGs between Late 1 and Late 2 fates ordered by pseudotime. H, I DDR tree visualization of FAP trajectory with mapping of pseudotime (H) and senescence annotation (I). J Plot showing the ss-GSVA score of cell cycle activity signature genes along FAP aging trajectory psuedotime with 95% CI. K Heat map visualization of expression levels of genes with correlated expression profiles to FAP aging pseudotime from Early to Late stage. L, M DDR tree visualization of EC trajectory with mapping of pseudotime (L) and senescence annotation (M). N Plot showing the ss-GSVA score of cell cycle activity signature genes along EC aging trajectory with 95% CI. O Heat map visualization of expression levels of genes with correlated expression profiles to EC aging pseudotime. P, Q DDR tree visualization of SMC trajectory with mapping of pseudotime (P) and senescence annotation (Q). R Plot showing the ss-GSVA score of cell cycle activity signature genes along SMC aging trajectory with 95% CIs. S Heat map visualization of expression levels of genes with correlated expression profiles to SMC aging pseudotime. Source data are provided as a Source Data file.