Table 3 List of antibodies used in the study
Target | Product ID | Catalog Number |
|---|---|---|
Viability dye | Fixable Blue Dead Cell Stain — LIVE/Dead™ (Thermo) | L34961 |
Flow count beads | Precision Count Beads (Biolegend) | 424902 |
B7H3-his | B7H3-his protein to detect CAR at the cell surface (R&D Biosystems) | 1949-B3-050/CF |
His | Anti-his mAb clone J095G46-APC (BioLegend) | 362605 |
Ag density quantification | BD Biosciences™ Quantibrite™ Phycoerythrin (PE) Beads; used to quantify B7H3 antigen density on target cell surface | 10626384 |
Fixation buffer | Fixation buffer (Biolegend) | 420801 |
Cell membrane dye | eBioscience Cell Proliferation Dye eFluor 450 (blue) (Thermo Fisher); used to label cells for BEHAV3D analysis | 65-0842-85 |
Cell membrane dye | Calcein AM (green) (Thermo Fisher); used to label cells for BEHAV3D analysis | C1430 |
Cell membrane dye | CellTrace™ Far Red Cell Proliferation Kit, for flow cytometry; used to label cells for Lumicks z-Movi analysis | C34564 |
RQR8 marker gene | Clone QBEND10-APC (BioTechne) | FAB7227A |
CD3 | Clone OKT-3-Brilliant Violet 711 (Biolegend) | 317328 |
CD4 | Clone VIT4-VioBlue (Miltenyi Biotec), | 130-113-219 |
B7H3 | Clone MIH42-PE (BioLegend) | 351003 |
PD-1 | Clone EH12.1-Brillant Violet 605 (Beckton Dickinson) | 563245 |
CD25 | Clone 2A3-Briljant Violet 711 (Beckton Dickinson) | 563159 |
CD4 | Clone A161A1-FITC (BioLegend) | 357406 |
CD8 | Clone SK1-PerCP (BioLegend) | 344706 |
TIM-3 | Clone F38-2E2-PE (BioLegend) | 345006 |
CCR7 | Clone G043H7-PE/Dazzle594 (BioLegend) | 353235 |
CD69 | Clone FN50-PE-Cy7 (Beckton Dickinson) | 561928 |
CD137 | Clone 4B4-1-APC (Beckton Dickinson) | 550890 |
CD3 | Clone HIT3a-Alexa Fluor 700 (BioLegend) | 300324 |
muCD45 | Clone 30-F11-APC/FireTM 810 (BioLegend) | 103173 |
CD45RA | Clone HI100-APC/Cy7 (Beckton Dickinson) | 750258 |
