Fig. 1: Exchange of BusA CDD/BusB NDD with SlnA7 CDD/SlnA8 NDD did not affect the butenyl-spinosyn biosynthesis. | Nature Communications

Fig. 1: Exchange of BusA CDD/BusB NDD with SlnA7 CDD/SlnA8 NDD did not affect the butenyl-spinosyn biosynthesis.

From: Reply to: Docking domains from modular polyketide synthases and their use in engineering

Fig. 1: Exchange of BusA CDD/BusB NDD with SlnA7 CDD/SlnA8 NDD did not affect the butenyl-spinosyn biosynthesis.

a The strategy used to substitute the CDD of BusA with that of SlnA7 and the NDD of BusB with that of SlnA8. b Butenyl-spinosyn A production by S. albus J1074 strains harboring gene clusters containing the wild-type or exchanged docking domain coding sequences. Statistical analysis was performed using GraphPad Prism 8.0.1 software and a two-tailed unpaired t-test. The p values less than 0.05 were considered significant. n = 3 independent fermentation samples. Data are presented as the mean ± S.D. ns not significant.

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