Fig. 5: M1-mutIL-18 promotes T cells differentiation.

a−d Single-cell RNA-seq data for about 15,000 CD45⁺ sorted cells. C57BL/6 J mice were implanted with B16F10 cells and treated with M1 (1.5 × 10⁷ PFU) or M1-mutIL-18 (1.5 × 10⁷ PFU) when tumor volumes were about 200 mm³. M1 or M1-mutIL-18 were administered via tail vein injection for 5 consecutive days. Mice were euthanized on the 3rd day after the last dose administration, and total RNA extracted from CD45⁺ sorted cells in tumors were detected by scRNA-seq and scTCR-seq. a Distribution of different cluster in CD45⁺ cells or lymphocytes. b Frequency of different cluster in CD45⁺ cells or lymphocytes. c Expression of co-inhibitory and co-stimulatory immune checkpoints and effector molecules in each cluster of CD8⁺ T cells. d Pseudo-time analysis of different cluster in CD8⁺ T cells.