Fig. 1: The vegetative phase change phenotype of GA biosynthesis mutants is not rescued by enhanced CK signaling.

a, c Number of leaves without abaxial trichomes of SD-grown rock2 ga1 (a) and rock2 ga3ox1,2 (c) hybrid plants in comparison to their respective parents and wild type. In box plots, the center line represents the median value and the boundaries indicate the 25th percentile (upper) and the 75th percentile (lower). The X marks the mean value. Whiskers extend to the largest and smallest value, excluding outliers which are shown as dots. b, d Length-to-width ratios of the blades of leaves 4 to 7. Data displayed are expressed as mean ± SEM of SD-grown plants. Numbers of biological replicates: (b) Col-0 (n4 = 32; n5 = 32; n6 = 33; n7 = 32), rock2 (n4 = 32; n5 = 31; n6 = 30; n7 = 32), ga1 (n4 = 38; n5 = 38; n6 = 38; n7 = 38), rock2 ga1 (n4 = 29; n5 = 29; n6 = 30; n7 = 30); (d) Col-0 (n4 = 28; n5 = 28; n6 = 29; n7 = 29), rock2 (n4 = 24; n5 = 26; n6 = 27; n7 = 27), ga3ox1 ga3ox2 (n4 = 30; n5 = 32; n6 = 32; n7 = 32), rock2 ga3ox1,2 (n4 = 32; n5 = 32; n6 = 31; n7 = 31). Asterisks indicate statistically significant differences compared to the wild type, as calculated by Mann-Whitney test (***p < 0.001) (a); letters indicate statistically significant differences between the genotypes, as calculated by one-way ANOVA, post-hoc Tukey’s test (p < 0.05) (b, d) or Kruskal-Wallis test (q < 0.05) (c).