Fig. 3: Expression of GA biosynthesis genes is downregulated in CK-deficient plants. | Nature Communications

Fig. 3: Expression of GA biosynthesis genes is downregulated in CK-deficient plants.

From: Cytokinin depends on GA biosynthesis and signaling to regulate different aspects of vegetative phase change in Arabidopsis

Fig. 3

a, b, d, e, g, h Expression of GA biosynthesis genes in whole shoots of SD-grown ahk2 ahk3 and CKX1ox plants compared to the wild type. Numbers of biological replicates: Col-0 (n7 = 6; n14 = 6; n21 = 6), CKX1ox (n7 = 6; n14 = 6; n21 = 4), ahk2 ahk3 (n7 = 5; n14 = 6; n21 = 5). Dots indicate each single biological replicate. c, f, i Expression kinetics of GA biosynthesis genes in 10-day-old SD-grown wild-type seedlings after treatment with 1 µM BA (n = 6 biological replicates). Transcript levels were determined by qRT-PCR. Data were normalized to TAFII15 and PP2AA2. Data displayed are expressed as mean ± SEM. Asterisks indicate statistically significant differences compared to the wild type of the respective time point (a, b, d, e, g, h) or compared to time point 0 (c, f, i), as calculated by one-way ANOVA, post-hoc Dunnett’s test (*p < 0.05; **p < 0.01; ***p < 0.001).

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