Fig. 1: GF BTBR do not manifest ASD-associated phenotypes.
From: Gut microbiota and brain-resident CD4+ T cells shape behavioral outcomes in autism spectrum disorder
A Representative heat maps and time spent in either the social or empty chambers during the three-chamber sociability test. SPF BTBR n = 18, GF BTBR n = 22. P value from two-way ANOVA, F1,76 = 1.948. B Representative heat maps and time spent in either the familiar or novel chambers during the three-chamber social novelty test. SPF BTBR n = 18, GF BTBR n = 22. P value from two-way ANOVA, F1,76 = 5.817. C Representative images and percent of marbles buried during the marble burying test. SPF BTBR n = 12, GF BTBR n = 18. P = 0.000002 from two-tailed Welch’s t-test, t(11.97) = 6.540. D Representative traces, time spent in the center zone, and frequency to the center zone during the open field maze. SPF BTBR n = 10, GF BTBR n = 12. P value from two-tailed Welch’s t-test, t(12.76) = 3.785 and t(18.95) = 0.7207, respectively. E Mean velocity and distance traveled during the open field maze. SPF BTBR n = 10, GF BTBR n = 12. P value from two-tailed Welch’s t-test, t(18.95) = 0.7207 and t(18.94) = 0.7229. F–H Representative immunofluorescence staining and number of c-FOS+ neurons per area in the amygdala (two-tailed Student’s t-test, t(5) = 3.562. SPF BTBR n = 5 sections, 3 mice, GF BTBR n = 7 sections, 4 mice) (G) and dentate gyrus of the hippocampus (t(10) = 3.769. SPF BTBR n = 6 sections, 3 mice, GF BTBR n = 6 sections, 3 mice) (H). All data represented as mean ± SEM. Source data are provided as source data file.
