Fig. 6: Healthy gut microbiota reduces ASD-associated metabolites in BTBR mice.
From: Gut microbiota and brain-resident CD4+ T cells shape behavioral outcomes in autism spectrum disorder
A Beta diversity from Principal coordinates analysis (PCoA) based on Bray-Curtis dissimilarity index demonstrating a unique microbiota composition following colonization with healthy gut microbiota from B6 mice (Pairwise PERMANOVA, F = 5.61, R2 = 0.38, P = 0.004). B LEfSe analysis of genera enriched in SPF BTBR or B6 microbiota recipient BTBR (BTBR-B6) mice. Genera with FDR-adjusted P value cutoff of 0.1 and Log LDA > 2.0 shown. C Volcano plot of fecal metabolites enriched in SPF BTBR or BTBR-B6 mice. Fold change threshold of 1.5 with two-tailed raw P value threshold of 0.05 from unpaired analysis with equal group variances. All significant metabolites labeled. D–F Relative glutamate (D), glutamate to GABA ratio (E), and 3-hydroxyglutaric acid (F) levels in feces after colonization with healthy gut microbiota. SPF BTBR n = 5, BTBR-B6 n = 6. P values from two-tailed Student’s t-test, t(9) = 2.299 (D), t(9) = 2.000 (E), t(9) = 2.466 (F). All data represented as mean ± SEM. Source data are available in source data file.
