Fig. 3: DVL1 is dependent on ETS1 to bind to promoter regions of ETS1 regulated genes.

a RT-qPCR (n  =  3 biological replicates (3 technical replicates each), mean ± SD, two-way, ANOVA followed by Dunnett’s multiple comparison test was used to test significance) of ETS1 regulated genes (n  =  3, log2FC  >  2 or < −2, padj < 0.05, statistical analysis and p-value calculations were performed using EdgeR),) identified in RNA/ChIPseq integrated analyses, in the presence (DVL1^OE and DVL1^+/-/-) or absence (DVL1^KD and DVL^-/-/-) of DVL1 against a non-targeted control cell line. b ChIP and ChIP/re-ChIP (n  =  3 biological replicates (3 technical replicates each), mean ± SD, two-way, ANOVA followed by Dunnett’s multiple comparison test was used to test significance) in MDA-MB-231, top, and HEK293TREx, bottom, with various degrees of expression of DVL1. c ETS1 regulated gene expression in ETS1 knockdown cells (n  =  3 biological replicates (3 technical replicates each), mean ± SD, two-way ANOVA, followed by Sidak’s multiple comparison test was used to test significance. d ChIP-qPCR (n  =  3 biological replicates (3 technical replicates each), mean ± SD, two-way, ANOVA, followed by Dunnett’s multiple comparison test was used to test significance) in MDA-MB-231, top, and HEK293TREx-TKO^+/-/-, bottom, control (ETS1^NTC) and ETS1 knockdown (ETS1^KD) cells. e Illustration showing DVL1 partners with ETS1 in the promoter region of ETS1 regulated genes (BMP2, CD96, COL1A1, DDX3X, PTEN, SOX4), top; Absence of DVL1 alters DVL1 binding ability to genomic loci but does not affect ETS1 binding, and changes gene expression of ETS1 regulated genes, bottom left; knockdown of ETS1 affects DVL1 ability to bind to shared genomic loci and alters gene expression. Created in BioRender. Martinez, D. (2025) https://BioRender.com/ebwkjhc. Source data are provided as the Source Data file.