Fig. 1: YY1⁺ macrophages accumulate in hypoxic tumor tissues.

a Schematic diagram showing the process of imaging mass cytometry (IMC) using prostate tissue. IMC of the indicated markers in prostate cancer (PCa) patient tumors (b) and para-cancerous tissues (c). Pathchs were defined as containing at least 10 cells with a maximum distance of 15 μm between cells involved. Adjacent regions were merged together for downstream analysis. The expression of the marker was normalized using z-score, and a threshold of 0.5 was selected. Dot plots showing the ratio of YY1⁺ or YY1⁻ macrophages and the relative YY1 expression of macrophages in HIF-1α⁺ or HIF-1α⁻ patches. The representative plot and analysis are based on 46 tumor samples and 26 para-cancerous tissues. Dot plots present the mean values ± SD, and the p values are calculated using a two-tailed t-test. The p values of left, middle, and right plots of (b) are 0.002, 0.160, and 0.009, respectively; The p values of left, middle, and right plots of (c) are 0.759, 0.798, and 0.820, respectively. *P < 0.05, ns P > 0.05. Source data are provided as a Source Data file. Mø macrophage.