Fig. 7: Construction and application of YY1-DbTACs@jetPEI and transgenic mice.
a Schematic diagram illustrating the mechanism of YY1-DbTACs-mediated degradation of YY1. b Western blot in THP-1 cells treated with YY1-DbTACs. c Western blot of ubiquitin following HIF-1α IP in hypoxia-induced THP-1 cells treated with YY1-DbTACs. d Experimental flow chart of subcutaneous tumorigenesis in mice using M2pep-YY1/NC-DbTACs@jetPEI. Each experimental group consisted of five male C57BL/6 mice (6–8 weeks). e Tumor volume changes over 21 days following subcutaneous tumor implantation. The lower panel presents the mean values ± SD at each time point. The p values were calculated based on the last time point between two datasets using a two-tailed t-test (n = 5 independent experiments, P < 0.001). *P < 0.05. f Schematic diagram illustrating the mechanism of YY1-DcTACs and its tetrahedral structure. g Confocal microscopy images showing the responsiveness of YY1-DcTACs-FAM/BHQ1 in the indicated cell lines. h Changes in tumor volume within 14 days after injection of YY1/NC-DcTACs via the tail vein in a mouse subcutaneous tumor model. The T lymphocytes were sorted by flow cytometry before testing changes in the ratio of CD3+CD8+ T cells. The lower panel presents the mean values ± SD at each time point. The p values were calculated based on the last time point between the two datasets (two-tailed t-test, P < 0.001). *P < 0.05. i Schematic diagram illustrating the construction of YY1 transgenic mice with myeloid conditional knockout and the establishment of a subcutaneous tumorigenesis model. j Subcutaneous tumor size in mice after 21 days of tumor formation (n = 5 independent experiments, P < 0.001). Five wild-type male C57BL/6J mice and five C57BL/6J-YY1em1Cflox male mice were used in the experiment (6–8 weeks old). k T lymphocytes were sorted by flow cytometry in the CD45-gated immune cells after grinding the subcutaneous tumors of transgenic mice into a cell suspension, and changes in the ratio of CD3+CD8+ T cells were observed (n = 5 independent experiments, P < 0.001). Bar graphs (h, j, k) present the mean values ± SD, and the p values are calculated using a two-tailed t-test. * P < 0.05. Source data are provided as a Source Data file.
