Fig. 5: The B cell characteristics by flow cytometry and single cell RNAseq.

a APS-1 (automatic population separator) of the flow cytometric data based on the expression of surface markers listed in the methods, Table ii, (a) and Supplementary Fig. 19). The square symbols indicate each individual, coloured by population, and the contour lines indicate 1 × Standard Deviation for each population. The dotted arrow indicates the direction of differentiatin and the solid arrow the direction of plasma cell differentiation. b B cell clusters identified using 10× single cell RNA-sequencing (cells from all time points with immunoglobulin gene removed). c Top (n = 2) genes defining each B cell cluster (cells from all time points). d Plasma cell proportion of total B cells at study time points (each coloured line is an individual). e The proliferating B cell proportion of B cells at study time points (each coloured line is an individual). f CDRH3 length in IgM plasma cells at study time points (day 1 n = 2 cells, day 11 n = 6 cells, day 64 n = 3 cells, day 92 n = 9 cells). The horizontal lines are the median and interquartile range (IQR) with whiskers to +/– 1.5 × IQR. g CDRH3 length in IgG plasma cells at study time points (day 1 n = 8 cells, day 11 n = 37 cells, day 64 n = 7 cells, day 92 n = 32 cells). The horizontal lines are the median and interquartile range (IQR) with whiskers to +/– 1.5 × IQR. h Hamming distance of CDRH3 sequences from plasma cells to known Ebola glycoprotein binding antibody sequences. The two plasma cell CDRH3 sequences closest to known GP binding monoclonal antibodies (within 3 amino acid changes) are highlighted with a blue arrow. A two-sided Wilcoxon rank-sum test was performed in (f and g).