Fig. 6: Pseudobulk analysis of the B cell single cell RNA-sequencing data. | Nature Communications

Fig. 6: Pseudobulk analysis of the B cell single cell RNA-sequencing data.

From: Prediction and characterisation of the human B cell response to a heterologous two-dose Ebola vaccine

Fig. 6: Pseudobulk analysis of the B cell single cell RNA-sequencing data.The alternative text for this image may have been generated using AI.

a First and second principal component (PC) of pseudobulk log10 counts per million expression values. b Volcano plot highlighting DEGs (false discovery rate [FDR] <0.05, red upregulated and blue downregulated) in plasma cells 10 days following the first study vaccine. c Volcano plot highlighting DEGs (FDR < 0.05) in plasma cells 7 days following the second study vaccine. d Gene set enrichment analysis on differentially expressed gene list from pseudobulk plasma cells 10 days following the first study vaccine compared with baseline, the most diffentially regulated upregulated and downregulated pathways are displayed (FDR < 0.05). e Gene set enrichment analysis on differentially expressed gene list from pseudobulk plasma cells 7 days following the second study vaccine compared with baseline, the most diffentially regulated upregulated and downregulated pathways are displayed (FDR < 0.05).

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