Fig. 7: Transarterial chemoembolization (TACE) using epirubicin-loaded 3Asphere (EPI-3Asphere).
a EPI encapsulation profiles in 3Asphere and CalliSpheres® with time (n = 3 biological samples). Statistical analysis was performed using unpaired two-tailed Student’s t-test. b EPI distribution in 3Asphere observed by CLSM. Cy5-3Asphere: Cy5-labeled 3Asphere (n = 3 independent experiments). Scale bars: 100 μm. c Drug loading efficiency (DLE) and DLC of EPI at different theoretical DLC (n = 3 biological samples). d Long-term storage stability of EPI-3Asphere by monitoring the sizes and DLE changes at 4 °C (n = 3 biological samples). e HAase and pH-dependent EPI release from 3Asphere (n = 3 biological samples). f Representative CT-enhanced images. Scale bars: 1 cm. g Relative tumor growth volume (n = 3 rabbits in each group). Statistical analysis was performed using unpaired two-tailed Student’s t-test. h Kaplan-Meier survival curves of tumor-bearing rabbits (n = 3 rabbits in each group). Statistical analysis was performed using a log-rank (Mantel-Cox) test (comparison between EPI-3Asphere and EPI-CalliSpheres®). i Representative CT images of tumor metastasis of lung tissues under different treatments. Scale bars: 1 cm. j Representative H&E staining slices of lung tissue at 14 days post-treatment (n = 3 independent experiments). Scale bars: 2 mm. k Tumor metastasis rate per unit area of lung tissues in H&E staining images (n = 3 biological samples). Statistical analysis was performed using unpaired two-tailed Student’s t-test. Data are presented as mean ± SD. Source data are provided as a Source Data file.
