Fig. 2: Microbubbles compositions and cell line influence microbubble uptake.
From: Imaging of macrophage accumulation in solid tumors with ultrasound
a Schematic representation of the different MB composition that we incubated with MΦs. “Created in BioRender. Kim (2025) https://BioRender.com/kqv3lyu”. b Quantification and c representative microscopy images of MΦ-labeling with Lipid MBs with different shell composition (mannose shell vs. lipid shell) and gas core (Octafluoropropane—C3F8—shown with “O” vs. Decafluorobutane—C4F10—shown with “D”) developed in house following 4 h of incubation. Scale bar 20 μm. (n = 4 petri dishes per group). Data are presented as mean values ± SD. p values were determined by one-way ANOVA followed by Tukey’s multiple comparisons. ****p < 0.0001; n.s. not significant. (p value for Lipid-O and Mannose-O is p = 0.9805 and Lipid-D and Mannose-D is p = 0.0533). d Representative microscopy images of MB-BMDMs. Scale bar 20 μm. e Quantification of MB uptake by BMDMs (n = 5 animals). Representative microscopy images of primary human macrophages (f) and dendritic cells (g) labeled with microbubbles. Scale bar 20 μm. Similar results were observed over three different blood samples for human macrophages and two different blood samples for dendritic cells. Source data are provided as a Source Data file.
