Fig. 6: NRBP2 represses innate immune response and NRBP2 mRNA level is negatively correlated with occurrence of RA.
From: Opposing roles of pseudokinases NRBP1 and NRBP2 in regulating L1 retrotransposition
a, b Pie charts illustrating the numbers of genes displaying significantly altered expression levels (fold change ≥ 1.5 and FDR < 0.05) following the knockdown of either NRBP2 (a) or NRBP1 (b) in HeLa cells. c Venn Diagram showing overlapping genes upregulated by NRBP2 knockdown in HeLa cells and genes activated upon L1 overexpression in RPE cells. p < 2.081 ×10−25, calculated using http://nemates.org/MA/progs/overlap_stats.html. d GO term analysis for the overlapping genes in (c) using DAVID. p values were calculated using a modified Fisher’s exact test (EASE score). Terms with –log₁₀p > 2 are shown. No correction for multiple comparisons. e NRBP2 knockdown upregulates multiple immune-related genes, partly in an L1-dependent manner. qRT-PCR was performed following NRBP2 knockdown alone or with L1 siRNA in HeLa cells. n = 3 biological replicates. Data are mean ± SEM. Two-sided unpaired t-test; no multiple comparison adjustment. n.s., not significant. * p < 0.05, * * p < 0.01, * * * p < 0.001. p values: IL32 (0.04, 0.0005), MMP19 (0.03, 0.008), IFIT1 (0.053, 0.24), DDX58 (0.02, 0.95), TLR1 (0.04, 0.97), CCL5 (0.08, 0.6), IL34 (0.08, 0.007), IFI44 (0.52, 0.26), ISG15 (0.81, 0.27), IFIT2 (0.24, 0.17), IL7R (0.047, 0.19). f mRNA levels of NRBP1 and NRBP2 in samples obtained from both healthy individuals (n = 28) and rheumatoid arthritis (RA) patients (n = 152). Box plots show the median (center line), the 25th and 75th percentiles (box bounds), and the whiskers extend to the minimum and maximum values, excluding outliers. Outliers are shown as individual points. Two-sided Wilcoxon rank-sum test. No correction for multiple comparisons. Data were retrieved from Autoimmune Diseases Explorer (https://adex.genyo.es).
