Fig. 6: Deficiency of ST2 in Treg cells leads to their retention in inguinal lymph nodes (LNs) at a precursor stage while reducing their numbers in the BM.
A Bulk RNA sequencing was performed, and clustering heatmap analysis was performed using selected differentially expressed genes related to precursor markers and non-lymphoid tissue activation markers in Treg cells derived from three different sources on day 14 post leukemic cell challenge (n = 3). B Statistically analyzed frequencies of NFIL3+ (n = 4), BATF+ (n = 5 for LN; n = 6 for BM), and GATA3+ (n = 5) Treg cells from both LN and BM tissues on day 14 post MLL-AF9 leukemic cell challenge (105 per mouse). Data are mean ± s.e.m.; unpaired t-test was used. C Comparisons of percentages of Treg cells for the indicated chemokine receptors between paired ST2+ Treg cells and ST2− Treg cells. CCR1 (n = 4), CCR2 (n = 4), CCR5 (n = 8), CCR6 (n = 6), CCR7 (n = 4), CCR8 (n = 4), CCR9 (n = 4), CXCR3 (n = 4), CXCR4 (n = 4) and CXCR6 (n = 4) post MLL-AF9 leukemic cell challenge (105 per mouse) on day 10. Data represents three independent replicates. The control group had no tumor cell transfer. A paired t-test was used.
