Fig. 2: Chromatin contact regions in human arterioles contain DNA regulatory elements and correlate with greater gene expression. | Nature Communications

Fig. 2: Chromatin contact regions in human arterioles contain DNA regulatory elements and correlate with greater gene expression.

From: Chromatin interaction maps of human arterioles reveal mechanisms for the genetic regulation of blood pressure

Fig. 2: Chromatin contact regions in human arterioles contain DNA regulatory elements and correlate with greater gene expression.

A Venn diagrams showing the overlap between loops identified in arterioles and EC-denuded arterioles (EDA) by Micro-C at 4 kb, 8 kb, and 16 kb resolutions. B Venn diagrams showing the overlap between interactions identified in arterioles and EDA by pan-promoter Capture Micro-C at 10 kb and 20 kb resolutions. C Bar plot showing the number of loops with regulatory elements present in one or both chromatin contact regions in arterioles and EDA across resolutions. “None,” “one,” and “both” indicate the presence of regulatory elements in neither, one, or both interacting regions, respectively. D Number of chromatin interactions classified by interaction type: promoter-promoter (PP), enhancer-promoter (EP), enhancer-enhancer (EE), enhancer-transcription factor binding site (ET), promoter-transcription factor binding site (PT), and transcription factor binding site-transcription factor binding site (TT). Categories are not mutually exclusive. E Expression levels of genes proximal to chromatin interactions (within 5 kb upstream or downstream of contact regions), grouped by interaction type (PP, EP, EE, ET, PT, TT). Controls include genes proximal to regulatory regions, including promoters, enhancers, or transcription factor binding sites, that were not in contact regions based on our data. Each box summarizes gene-level data. The number of genes (n) per category varies and is provided in the Source Data file. Boxes represent the interquartile range (IQR, 25th to 75th percentile), center line shows the median, and whiskers extend to 1.5 × IQR. F Boxplot showing gene expression levels grouped by the number of chromatin interactions involving gene promoter regions. Spearman correlation was applied. Each box summarizes gene-level data. The number of genes (n) per category is provided in the Source Data file. Boxes represent the IQR (25th to 75th percentile), center line shows the median, and whiskers extend to 1.5 × IQR.

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