Fig. 6: Deletion of a 4-bp noncoding genomic segment containing the rs1882961 orthologous site attenuates salt-induced hypertension in the Dahl salt-sensitive (SS) rat.
A Comparative mapping and deletion of a 4-bp rat genomic segment containing the rs1882961 orthologous site. See Supplementary Fig. S11 for additional detail and confirmation of deletion. The image at the top was generated using the UCSC Genome Browser, http://genome.ucsc.edu. The deletion of the 4-bp noncoding genomic segment in the SS rat attenuated salt-induced hypertension (B–G). B Mean arterial pressure (MAP). C Systolic blood pressure (SBP). D Diastolic blood pressure (DBP). E Pulse pressure. F Heart rate. G Hourly averages of MAP on days 12 to 14 on a 4% NaCl high salt diet. WT wild-type littermate SS rats, Δrs961 SS-Δrs1882961−/− rats. For B–G, n = 7 for WT and 8 for Δrs961. Data are presented as mean ± SEM. #, p < 0.05, ##, p < 0.01 for WT vs. Δrs961 by two-way RM ANOVA; *, p < 0.05 vs. WT by Holm-Sidak test. H Nrip1 expression in the mesentery was lower in SS-Δrs1882961−/− rats compared to WT. In normal salt, n = 7 for WT and n = 9 for Δrs961; in high salt, n = 4 for WT and n = 5 for Δrs961. Data are presented as Mean ± SEM. *, p < 0.05, Two-way ANOVA followed by Holm-Sidak test. I Vascular smooth muscle sensitivity to nitric oxide (NO) in the 3rd order mesenteric artery was improved in SS-Δrs1882961−/− rats compared to WT. Spermine NONOate, an NO donor. N = 6 for WT and 7 for Δrs961 rats. Data are presented as mean ± SEM. ##, p < 0.001 for WT vs. Δrs961; *, p < 0.001 for 10-7 and 10-6 M and p = 0.019 for 10-5 M; two-way RM ANOVA, followed by Holm-Sidak test.
