Fig. 1: Overview of MacroMap.

Overview of study: a Genotyped iPSC cell lines were differentiated into macrophages, and RNA was harvested before differentiation (Prec_D0) and 2 days after starting differentiation (Prec_D2). RNA was also harvested from differentiated macrophages at 6 and 24 h (Ctrl_6 and Ctrl_24). Naive macrophages were then exposed to a panel of 10 stimuli and RNA was harvested at 6 and 24 h after stimulation. b Split reads were used to quantify intron usage ratios on an individual level using LeafCutter. Split reads were then used for differential splicing analysis between naive and stimulated conditions, and as a quantitative trait to map splicing quantitative trait loci (sQTLs). sQTLs were then colocalised with 22 immune-mediated disease GWAS summary statistics.