Fig. 5: Hypoxic chemotactic property of FeS@SRB in vitro. | Nature Communications

Fig. 5: Hypoxic chemotactic property of FeS@SRB in vitro.

From: Living therapeutics of nonpathogenic bacteria as biosynthesis factory and active carriers for enhancing tumor-targeted therapy

Fig. 5: Hypoxic chemotactic property of FeS@SRB in vitro.

A Schematic diagram of the transwell system that simulates the hypoxic chemotaxis of FeS@SRB (OD600 = 0.1) in vitro. B Representative fluorescence images in the upper and lower chambers of FeS@SRB at different time points under hypoxic environment. Scale bar: 50 μm. C Corresponding FI ratio based on fluorescence images. n = 3 independent samples. Data are presented as mean ± SD. Source data are provided as a Source Data file. D Confocal fluorescence images and (E) 3D confocal fluorescence images of 4T1 multicellular 3D spheroids co-incubated with FITC-labeled FeS@SRB for 24 h. Scale bar: 200 μm. F Confocal fluorescence micrographs and (G) 3D confocal fluorescence images of B16F10 multicellular 3D spheroids co-incubated with FITC-labeled FeS@SRB for 24 h. Scale bar: 200 μm. The schematic diagram was created in BioRender. Dong, M. (2025) https://BioRender.com/k7shv52.

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