Fig. 1: The geographic distribution of gastrointestinal helminths detected by sequencing among worm or egg isolates and faecal samples analysed.

World maps show the countries of origin for all samples used in the study, which include (a) faecal samples (n = 842) from 15 countries and (b) worm and concentrated egg isolates (n = 158) from 15 countries. The point size on the maps indicates the number of samples from each location. Heatmaps display the relative prevalence of single and mixed-species infections of parasites detected by sequencing from (c) faecal samples and (d) worm/concentrated egg isolates, respectively. The faecal sample icon indicates faecal samples and adult worm/egg figures indicate samples from adult worms and/or concentrated worm eggs. A minimum normalised coverage threshold of 10 reads was applied, resulting in 329 samples from 27 countries reported as positive by sequencing for at least one parasite. Genomic datasets from faecal samples were plotted separately from the worm or egg datasets using a different scale based on the obtained coverage. Colours reflect read counts, normalised by the total number of reads per sample per genome size, to achieve ‘reads mapped per million reads per Mb’. Sample site abbreviations for both faecal and worm data are as follows: ARG = Argentina; BEN = Benin; BGD = Bangladesh; CHN = China; CMR = Cameroon; DRC = Democratic Republic of the Congo; ECU = Ecuador; ETH = Ethiopia; FJI = Fiji; GLP = Guadeloupe; HND = Honduras; IND = India; KEN = Kenya; LKA = Sri Lanka; MMR = Myanmar; MOZ = Mozambique; MWI = Malawi; MYS = Malaysia; NGA = Nigeria; PRI = Puerto Rico; SEN = Senegal; THA = Thailand; TZA = Tanzania; UGA = Uganda; USA = United States of America; ZAF = South Africa. Faecal, worm, and egg icons are provided by Servier Medical Art (https://smart.servier.com/), licensed under CC BY 4.0 (https://creativecommons.org/licenses/by/4.0/). Source data are provided as a Source Data file.