Fig. 1: LoopTrace enables high precision 3D DNA tracing in non-denatured cells. | Nature Communications

Fig. 1: LoopTrace enables high precision 3D DNA tracing in non-denatured cells.

From: Nanoscale 3D DNA tracing in non-denatured cells resolves the Cohesin-dependent loop architecture of the genome in situ

Fig. 1

Maximum projection of RPE-1 cells labelled with DAPI (grey) and oligoFISH probes (different genomic regions are indicated with pseudocolors), individual spots during 30 exchange cycles with centroid fits, projection of sequential imaging steps and centroid fits, and resulting 3D DNA trace. ID of Individual tracing spots is equivalent to barcode ID in Supplementary Fig. S1a, b. Data representative of n = 1561 cells from 2 independent experiments. Scale bar in overlay 200 nm, for DNA-trace 100 nm.

Back to article page