Fig. 1: Metabolic networks of DS-TB and DR-TB clinical isolates altered by trehalose supplementation. | Nature Communications

Fig. 1: Metabolic networks of DS-TB and DR-TB clinical isolates altered by trehalose supplementation.

From: Trehalose catalytic shift inherently enhances phenotypic heterogeneity and multidrug resistance in Mycobacterium tuberculosis

Fig. 1

A 2D (left panel) and 3D (right panel) principal component analysis (PCA) of metabolome profiles from DS- (red), RSR- (green), MDR- (blue), XDR- (magenta), and TDR-TB (light blue) clinical isolates cultured in m7H9 containing 20 mM trehalose. B Targeted metabolomics analysis focusing on intermediates in trehalose metabolism, glycolysis, and the pentose phosphate pathway. PEP, phosphoenolpyruvate. Data points represent mean values ± s.e.m. of 15 biological replicates and p-values were determined by one-way ANOVA with Bonferroni post-test correction (ns, not significant). IC ion counts. C qRT-PCR analysis of treS expression in TB clinical isolates. Data points represent mean values ± SD of 15 biological replicates and p-value was determined by Student’s unpaired t-test with Welch’s correction. Source data are provided as a Source Data File.

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