Fig. 1: Introduction to a new food reduces self-brood size and causes germ cell dysfunction.
From: C. elegans sperm and oocytes differentially transmit diet-induced adaptations to their progeny
a Schematic of the experimental design. Bleached embryos from OP-adapted hermaphrodites were placed on plates seeded with the indicated bacteria. Ten individual G0 L4 hermaphrodites were then individually plated on 10 separate plates for each of the four foods. At each subsequent generation, a single early-brood L4 hermaphrodite was selected as the founder of the next generation. All progeny on all plates were counted. b Quartile box plots (whiskers extend up to 1.5* interquartile range (IQR)) of self-brood size of G1 wild-type (N2) hermaphrodites previously adapted to growth on OP and newly transferred to the indicated bacterial foods (OP n = 50, BS n = 38, SM n = 40, PB n = 39). P values were calculated by Welch’s 2-sided t-test. c The percentage of 50 embryos from G0 (n = 5) and G10 (n = 5) hermaphrodites grown on each food that hatched. One-tailed t-test compared to OP grown worms. d The percentage of 10 self-sperm-depleted G0 (n = 3) or G10 (n = 2) hermaphrodites grown on the indicated food that produced cross-progeny when mated with OP grown wild-type males. In one G0 SM and one G10 BS experiment only 9 hermaphrodites were assayed. P values were calculated by one-tailed t-test compared to OP grown worms. e The percent of self-progeny males from the sum of all progeny (inset numbers) produced by five G0 or G10 animals grown on the indicated food. P values were calculated by Fisher exact test, compared to OP grown worms. Approximate statistical significance is indicated on some panels. * < 0.05, ** < 0.01, *** < 0.001, **** < 0.0001. Source data and numerical P values are provided as a Source Data file.
