Fig. 5: Experimental infection of chickens with bovine HPAI A(H5N1) 2.3.4.4b viruses.

Chickens (6 weeks old) were inoculated intra-tracheally with 106 EID50/0.3 mL of goose/KS/930F (H5N1) or bovine/OH/439 (H5N1) viruses (n = 6/group). After 16 hpi, two donor chickens from each experimental group were placed in contact with three naive direct contact chickens. The chickens were monitored twice daily for disease symptoms and survival.