Fig. 2: Screening of transcription factors (TFs) that are predicted to bind distNC-localized trDARs opening under reduced IIS reveals LIN-39 as a driver of longevity. | Nature Communications

Fig. 2: Screening of transcription factors (TFs) that are predicted to bind distNC-localized trDARs opening under reduced IIS reveals LIN-39 as a driver of longevity.

From: LIN-39 is a neuron-specific developmental determinant of longevity in Caenorhabditis elegans with reduced insulin signaling

Fig. 2

a The ten most significant enrichments of TF DNA-binding motifs from the Cis-BP database (build 2.00) in trDARs that open under reduced IIS. b The ten most significant enrichments of TF ChIP-seq peaks from modENCODE in trDARs that open under reduced IIS. c Screen for lifespan phenotypes of selected candidates from (a) and (b). Candidate TFs were knocked down by RNAi from the L1 stage in eri-1(mg366) and daf-2(e1370); eri-1(mg366) mutant C. elegans. “#1” and “#2” denote distinct RNAi clones targeting the same respective gene. d Kaplan–Meier survival estimates with 95% confidence intervals for eri-1(mg366) and daf-2(e1370); eri-1(mg366) mutant C. elegans grown from the L1 stage on the indicated RNAi bacteria. (n = 445 (eri-1; control RNAi), 272 (eri-1; lin-39 RNAi), 720 (daf-2; eri-1; control RNAi), 409 (daf-2; eri-1; lin-39 RNAi); for results from a second independent biological replicate experiment, see Supplementary Table S10). e Kaplan–Meier survival estimates with 95% confidence intervals for wild-type (N2) C. elegans and the same animals carrying an overexpression construct of LIN-39. (n = 211 (wild-type), 367 (lin-39 OE); for results from a second independent biological replicate experiment, see Supplementary Table S10). Boxes state changes in median lifespan and the associated p-values (based on two-sided log-rank tests) for the indicated comparisons. OE overexpression. Source data are provided as a Source Data file.

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