Table 1 Thermodynamic parameters for the binding of Retromer and DENND4C by ITC
From: Mapping of endosomal proximity proteomes reveals Retromer as a hub for RAB GTPase regulation
Kd (μM) | ΔH (kcal/mol) | ΔG (kcal/mol) | -TΔS (kcal/mol) | |
|---|---|---|---|---|
hRetromer | ||||
hDENND4C1189-1206 | 15.4 ± 0.85 | − 10.1 ± 1.41 | − 6.6 ± 0.04 | − 3.5 ± 1.44 |
hDENND4C1189-1206 L1204A | No binding detected | |||
hDENND4C1189-1206 + RT-D1 | No binding detected | |||
