Fig. 3: GDF11-specific aptamer did not recognize GDF11/8 mature ligand in the presence of their inhibitors.
From: Activated GDF11/8 subforms predict cardiovascular events and mortality in humans
A, B Anti-GDF11/8 mature ligands western blot of GDF8 (A) and GDF11 (B) mature ligands with inhibitors. The input shows the proteins before the pull-down. The proteins after the pull-down are below the “pulled-down sample” labeling, with non-pulled-down proteins on the left. Mature ligands and inhibitors are mixed in 1:2.5 molar ratio respectively. C Summary of ligand states captured by the aptamers. Experiments were independently repeated twice with similar results. GDF11/8 ligand (monomer): 12.4 kDa, GDF11/8 ligand (dimer): 25 kDa, FS288 = follistatin 288; FSTL3 = follistatin-like 3; WFIKKN1 = WAP, Kazal, immunoglobulin, Kunitz and NTR domain-containing protein 1; WFIKKN2 = WAP, Kazal, immunoglobulin, Kunitz and NTR domain-containing protein 2. Experiments performed in duplicate. Source data are provided as a Source Data File.
