Fig. 2: The free energy (FE) landscape of WT and EGFRex20ins mutations.

a WT EGFR, (b) near-loop insertion mutants (S768dupSVD and A767dupASV), and (c) far-loop insertion mutants (H773insNPH and H773insAH). The prominent FE basins are marked as states (I–IV), and the dashed black lines denote the most probable transition path between the inactive and the active state. States I and II predominantly correspond to a Src-like inactive state and the active state, respectively, while states III and IV are the intermediate states.