Fig. 4: The frd HT insertion mutations enhance M. bovis pathogenicity. | Nature Communications

Fig. 4: The frd HT insertion mutations enhance M. bovis pathogenicity.

From: Mycobacterium bovis frd operon phase variation hijacks succinate signaling to drive immunometabolic rewiring and pathogenicity

Fig. 4: The frd HT insertion mutations enhance M. bovis pathogenicity.

a–f C57BL/6 mice were infected with ~5000 CFU of M. bovis intranasally for 12 and 22 days. a Body weight gain rate curves in C57BL/6 mice uninfected or infected with M. bovis. b, c The ratio of lung or spleen weight to the total body weight at 12 and 22 dpi. d, e Bacterial loads in lungs or spleen. f The percentage of lung’s area occupied by inflammatory lesions. g HE-stained lung sections at 12 and 22 dpi. Arrows indicate lymphocytes, and arrowheads indicate neutrophils. Data are presented as mean values  ± SD. Sample size of n  =  5 mice was included in each group. P values depicted on the graphs were assessed using one-way ANOVA with Tukey’s multiple comparisons test. WT, wild-type. Scale bars, 1 mm (top) and 20 μm (bottom). Source data are provided as a Source Data file.

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