Fig. 6: Restitution of the fertility and JA/JA-Ile levels of opr2opr3 plants by OPR3-targeted to different organelles.

a–h Open flower buds showing stamen filament elongation and pollen release by anthers (scale bars represent 0.5 mm) and plant inflorescences showing silique formation at days 50-60 post germination with asterisks marking fully developed siliques. Col-0 and opr2opr3 transformed with an empty vector (a, b) show fertility and sterility, respectively. opr2opr3 T2 lines complemented with peroxisome- (c), nucleus- (d), cytosol- (e), ER- (f), plastid stroma- (g) and mitochondria- (h) targeted OPR3 fused to YFP show the rescue of the opr2opr3 sterility phenotype. Red asterisk show exemplary siliques containing seeds. i OPDA, JA and JA-Ile accumulation at 1 h after wounding of seedlings of wild-type (WT) or opr2opr3 transformed with either empty vector (EV) or 35S::OPR3 targeted to peroxisomes (Px), cytosol (Cyt) or mitochondria (Mt). Numbers refer to independent transgenic lines, which were selected for single insertion and homozygosity. Bars represent means of four biological replicates with 120 seedlings each (single dots; ±SEM). Statistically significant differences between opr2opr3 transformed with empty vector and transformed with the OPR3 constructs were calculated using One-Way ANOVA followed by Tukey HSD (p < 0.05) and are denoted by different letters. Source data are provided as a Source Data file.