Fig. 3: Spontaneous ADPr signal upon loss of ARH3 is sufficient to increase nucleosome dynamics. | Nature Communications

Fig. 3: Spontaneous ADPr signal upon loss of ARH3 is sufficient to increase nucleosome dynamics.

From: Single nucleosome imaging reveals principles of transient multiscale chromatin reorganization triggered by histone ADP-ribosylation at DNA lesions

Fig. 3: Spontaneous ADPr signal upon loss of ARH3 is sufficient to increase nucleosome dynamics.

A Western blot displaying APDr signal, stained with a pan-ADPr antibody as well as PARP1 and ARH3 signals in WT or ARH3 KO cells, left untreated or after 24 to 72 h of PARGi treatment (25 µM PDD00017273). H3 is used as loading control (n = 3). B Representative examples of the immobile population of H2B trajectories inside the nucleus of undamaged WT and ARH3 KO cells. C Mean jump distance KDE plots for the immobile population of H2B tracks in undamaged WT and ARH3 KO cells, treated or not with 25 µM PDD00017273 PARGi for 24 h. Total number of cells analyzed in at least three independent experiments (N). WT N = 35, 13769 trajectories; ARH3 KO N = 55, 81246 trajectories; ARH3 KO + PARGi N = 22, 23985 trajectories. Mean jump distance between each condition versus WT condition are significantly different (p < 0.001, two-sided Yuen–Welch test). Created in BioRender. Garcia Fernandez, F. (2025) https://BioRender.com/5akfy1x.

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