Fig. 5: The removal of MAR marks by ARH3 is needed for the recovery of the chromatin state following its initial relaxation at sites of damage.
A Representative confocal images and recruitment kinetics of the GFP-tagged WWE domain of RNF146 (PAR sensor) and Macrodomain of Macro D2 (MAR sensor) expressed WT and ARH3 KO U2OS cells after irradiation at 405 nm. Scale bars: 4 µm (PAR sensor NWT = 12, NKO = 12; MAR sensor NWT = 11, NKO = 12, mean ± SEM.) B Representative confocal images and relative average thickness of the photo-activated damaged area in WT and ARH3 KO cells expressing H2B-PAGFP and irradiated at 405 nm. Scale bars: 2 µm. Curves of the average thickness of the photo-activated line are mean ± SEM (WT N = 12, ARH3 KO N = 16). C Mean jump distance KDE plots for the immobile population of H2B tracks inside the irradiated region, before and at different times after micro-irradiation at 355 nm in WT and ARH3 KO U2OS cells. Total number of cells analyzed in three independent experiments (N). WT Nbef = 23, 847 trajectories total, N1min = 13, 1643 trajectories, N10min = 7, 603 trajectories; ARH3 KO Npre = 21, 1624 trajectories; N1min = 21, 1409 trajectories; N10min = 7, 357 trajectories. Created in BioRender. Garcia Fernandez, F. (2025) https://BioRender.com/5akfy1x.
